The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.)

Cong Cong Yang, Jian Ma, Cong Li, Min Sun, Ya Ya Zou, Ting Li, Yang Mu, Hua Ping Tang and Xiu Jin Lan
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Cong Cong Yang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Jian Ma: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Cong Li: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Min Sun: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Ya Ya Zou: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Ting Li: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Yang Mu: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Hua Ping Tang: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China
Xiu Jin Lan: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, P.R. China

Czech Journal of Genetics and Plant Breeding, 2020, vol. 56, issue 2, 52-61

Abstract: Thousand-grain weight (TGW) is an important trait affecting wheat production. We previously identified a major quantitative trait loci (QTL) controlling the TGW on the 2D chromosome of wheat using a recombinant inbred line (RIL) population constructed by the cross between Tibetan semi-wild wheat Q1028 (Q1028) and Zhengmai 9023 (ZM9023). The positive allele at this QTL is from ZM9023. To further characterise this QTL, here, we try to develop and validate the high-resolution melting (HRM) and sequence-characterised amplified region (SCAR) markers. One HRM marker (0C98-411) and two SCAR markers (E301-700 and B0BB-10470) were developed and integrated into the genetic map. All of these three markers were validated in three populations with different genetic backgrounds. 0C98-411 is the most closely linked marker that could trace QTgw.sau-2D in molecular marker assisted breeding.

Keywords: high-resolution melting marker; marker development; QTgw.sau-2D; sequence-characterised amplified region marker (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:56:y:2020:i:2:id:35-2019-cjgpb

DOI: 10.17221/35/2019-CJGPB

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