Discrimination of tobacco cultivars using SCAR and RAPD markers

Jiuzhe Sun, Junting Wang, Dongying Su, Jinchu Yang, Erbin Wang, Shixi Wu, Meng Li and Lin Ma
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Jiuzhe Sun: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China
Junting Wang: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China
Dongying Su: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China
Jinchu Yang: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China
Erbin Wang: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan, P.R. China
Shixi Wu: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China
Meng Li: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China
Lin Ma: School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, P.R. China

Czech Journal of Genetics and Plant Breeding, 2020, vol. 56, issue 4, 170-173

Abstract: Tobacco genetic purity is crucial to maintain the quality of cigarette products in the tobacco industry. To reduce the difficulties in the discrimination of large number of tobacco cultivars in production practice, we developed a two-step identification strategy by using SCAR and RAPD markers. A total of 53 tobacco cultivars were examined in the study. Initially, all the selected cultivars were divided into four groups, each group consisted of seven to seventeen tobacco cultivars based on difference in phenotypes identified by the SCAR markers S4 and S8. Later, in each group, each tobacco cultivar was identified using RAPD fingerprinting by using one to four polymorphic primers, which were selected from 200 random primers. The results showed that all 53 tobacco cultivars could be effectively distinguished by using only two SCAR and seven RAPD markers. The two-step fingerprinting strategy could be used as a convenient and cost-effective tool to discriminate large numbers of tobacco cultivars for production planning in the tobacco industry.

Keywords: cultivar; fingerprinting; identification; RAPD; SCAR; Nicotiana tabacum (search for similar items in EconPapers)
Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:56:y:2020:i:4:id:120-2019-cjgpb

DOI: 10.17221/120/2019-CJGPB

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