Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen in mice

Youngmin Park, Hyangju Kang, Kyungmin Min, Nam Hyung Kim, Minhee Park, In-Ohk Ouh, Ha-Hyun Kim, Jae-Young Song, Dong-Kun Yang, Eun-Ju Sohn and Sangmin Lee
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Youngmin Park: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea
Hyangju Kang: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea
Kyungmin Min: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea
Nam Hyung Kim: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea
Minhee Park: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea
In-Ohk Ouh: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea
Ha-Hyun Kim: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea
Jae-Young Song: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea
Dong-Kun Yang: Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, Korea
Eun-Ju Sohn: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea
Sangmin Lee: BioApplications Inc., Pohang Techno Park Complex, Pohang, Korea

Czech Journal of Genetics and Plant Breeding, 2021, vol. 57, issue 1, 26-35

Abstract: Rabies remains an infectious disease among humans and animals, and requires the development of an effective vaccine essential to prevent rabies. Advances in molecular biology and biotechnology have led to the development and improvement of many rabies vaccines. Before the third-generation of the vaccine, rabies vaccines were based on the virus itself. Thus, even if effective, these vaccines may not be completely safe, resulting in a strong demand for the development of effective subunit vaccines that do not raise concerns about virus replication and infection in the host. This study investigated the ability of the glycoprotein of the rabies virus to be expressed in tobacco plants (Nicotiana benthamiana) and to induce an immune response in mice. Using a transient transfection, a soluble glycoprotein was successfully expressed in N. benthamiana. Fusing of five histidine residues at the C-terminus enabled the glycoprotein to be easily purified by affinity chromatography. The glycoprotein expressed in the plants was found to be N-glycosylated post-translationally, and the mice immunised with this glycoprotein generated neutralising antibodies against the rabies virus. These results suggest that a glycoprotein produced in the endoplasmic reticulum of N. benthamiana is bioactive, and might be used to generate a subunit vaccine against the rabies virus.

Keywords: glycoprotein; molecular pharming; plant-made pharmaceuticals; subunit vaccine; rabies (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:57:y:2021:i:1:id:25-2020-cjgpb

DOI: 10.17221/25/2020-CJGPB

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