Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

Zhijun Tong, Sanjie Jiang, Weiming He, Xuejun Chen, Lixin Yin, Dunhuang Fang, Yafei Hu, Fangchan Jiao, Chi Zhang, Jianmin Zeng, Xinfu Wu, Shancen Zhao, Jianbo Jian and Bingguang Xiao
Additional contact information
Zhijun Tong: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
Sanjie Jiang: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Weiming He: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Xuejun Chen: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
Lixin Yin: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Dunhuang Fang: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
Yafei Hu: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Fangchan Jiao: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
Chi Zhang: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Jianmin Zeng: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
Xinfu Wu: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China
Shancen Zhao: BGI Institute of Applied Agriculture, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Jianbo Jian: BGI Genomics, BGI-Shenzhen, Shenzhen, Guangdong, P.R. China
Bingguang Xiao: Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, P.R. China

Czech Journal of Genetics and Plant Breeding, 2021, vol. 57, issue 3, 102-112

Abstract: Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

Keywords: crossing; single nucleotide polymorphism (SNP); whole genome sequencing (WGS) (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:57:y:2021:i:3:id:8-2021-cjgpb

DOI: 10.17221/8/2021-CJGPB

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