Study on fine mapping of QTL for juice yield traits of sweet sorghum (Sorghum dochna)

Bo Zhao, Buxian Xia, Jianming Gao, Feng Luo, Qiuling Chen, Jianpeng Lv, Oujing Li, Jingwang Li, Xiaopeng Tong, Huifen Liu, Shoujun Sun and Zhongyou Pei
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Bo Zhao: Tianjin Agricultural University, Tianjin, P.R. China
Buxian Xia: Tianjin Agricultural University, Tianjin, P.R. China
Jianming Gao: Tianjin Agricultural University, Tianjin, P.R. China
Feng Luo: Tianjin Agricultural University, Tianjin, P.R. China
Qiuling Chen: Tianjin Agricultural University, Tianjin, P.R. China
Jianpeng Lv: Tianjin Agricultural University, Tianjin, P.R. China
Oujing Li: Tianjin Agricultural University, Tianjin, P.R. China
Jingwang Li: Tianjin Agricultural University, Tianjin, P.R. China
Xiaopeng Tong: Tianjin Agricultural University, Tianjin, P.R. China
Huifen Liu: Tianjin Agricultural University, Tianjin, P.R. China
Shoujun Sun: Tianjin Agricultural University, Tianjin, P.R. China

Czech Journal of Genetics and Plant Breeding, 2022, vol. 58, issue 2, 55-63

Abstract: The stem juice yield is a key factor that influences both the biological and economic production of sweet sorghum [Sorghum dochna (Forssk.) Snowden]. To elucidate upon the genetic basis of the stem juice yield, an F5 population developed from a cross between the low juice yielding Xinliang52 (XL52) and high juice yielding W455 lines, were used in a quantitative trait locus (QTL) analysis. A main effect of the QTL controlling stem juice yield was separated with an SSR marker called Xtxp97, which explained 46.7% of the phenotypic variance. In addition, F5 and F6 populations were constructed with XL52 and W452 as the parents to further verify the QTLs, and a significant correlation was found between the juice yield trait and the Xtxp97 marker. Based on the progeny tests of 29 recombinants, QJy-sbi06 was located in a region of about 21.2 kb on chromosome 6, where a candidate gene encoding an NAC transcription factor (sobic.006G147400) was identified. Combining the different population association analysis and sequencing technology showed that XL52 inserted a 1.8 kb transposon in the NAC to directly interrupt and inactivate the juice yield gene. This study also demonstrated that the colour of the leaf midribs was controlled by a single gene and was significantly positive correlated with juiciness (r = 0.784, P < 0.01). These results could lay the foundation for map-based cloning of QJy-sbi06 and provide genes or QTLs for breeding sorghum lines with a high juice yield and quality.

Keywords: juice yield; NAC; QTL; Sorghum dochna; transposon (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:58:y:2022:i:2:id:48-2021-cjgpb

DOI: 10.17221/48/2021-CJGPB

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