Genomic SSR marker development in lentil (Lens culinaris Medik.) and assessment of cross-species/genera transferability to related legumes

Melike Bakir, Şehriban Demir, Cebrail Yildirim and Abdullah Kahraman
Additional contact information
Şehriban Demir: Department of Agricultural Biotechnology, Faculty of Agriculture, Erciyes University, Kayseri, Türkiye
Cebrail Yildirim: Institute of Hemp Research, Yozgat Bozok University, Yozgat, Türkiye
Abdullah Kahraman: Department of Field Crops, Faculty of Agriculture, Harran University, Sanliurfa, Türkiye

Czech Journal of Genetics and Plant Breeding, 2024, vol. 60, issue 1, 42-49

Abstract: Simple sequence repeat (SSR) markers are valuable genetic and genomic research tools and are extensively used in major crops. However, a paucity of available molecular markers in lentils (Lens culinaris Medik.) has significantly hindered genomic studies in this vital legume crop. In this study, we developed 33 new SSR markers for lentils using an enriched genomic library and tested their polymorphism in 10 lentil cultivars. We found that 16 (48.5%) SSR markers were polymorphic. The expected heterozygosity values of the polymorphic SSR markers ranged from 0.095 to 0.820, while observed heterozygosity values varied between 0.100 and 1.00. In addition, we tested the transferability of 86 SSR markers, including the 33 newly developed ones, to other legume species. Transferability rates of lentil SSR markers to other species varied between 13.76% (Vicia sativa L.) and 2.58% (Phaseolus vulgaris L. and Trifolium pratense L.). These new SSR markers could be used in further molecular breeding, population genetics, and genetic mapping studies in lentils and other legume species.

Keywords: cross-transferability; genomic library; legumes; simple sequence repeats (search for similar items in EconPapers)
Date: 2024
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjgpb.agriculturejournals.cz/doi/10.17221/57/2023-CJGPB.html (text/html)
http://cjgpb.agriculturejournals.cz/doi/10.17221/57/2023-CJGPB.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:60:y:2024:i:1:id:57-2023-cjgpb

DOI: 10.17221/57/2023-CJGPB

Access Statistics for this article

Czech Journal of Genetics and Plant Breeding is currently edited by Ing. Markéta Knížková, (Executive Editor)

More articles in Czech Journal of Genetics and Plant Breeding from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().