Impact of selected antimitotic substances on doubled haploid and polyploid regeneration in microspore cultures of swede (Brassica napus ssp. napobrassica (L.) Hanelt)

Miroslav Klíma, Yamen H. Shmeit, Pavel Kopecký, Pavel Vítámvás, Klára Kosová, Ilja T. Prášil and Eloy Fernández-Cusimamani
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Yamen H. Shmeit: Crop Research Institute, Prague-Ruzyně, Czech Republic
Pavel Kopecký: Centre of the Region Haná for Biotechnological and Agricultural Research, Crop Research Institute, Olomouc, Czech Republic
Pavel Vítámvás: Crop Research Institute, Prague-Ruzyně, Czech Republic
Klára Kosová: Crop Research Institute, Prague-Ruzyně, Czech Republic
Ilja T. Prášil: Crop Research Institute, Prague-Ruzyně, Czech Republic
Eloy Fernández-Cusimamani: Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic

Czech Journal of Genetics and Plant Breeding, 2024, vol. 60, issue 2, 79-85

Abstract: Several antimitotic agents were tested in three embryogenic doubled haploid (DH) lines of swede (Brassica napus ssp. napobrassica). No effect on embryogenesis was observed at the given concentrations of colchicine (5 μmol/L or 50 mg/L) and treatment time of 24 hours. Flow cytometric analysis of microspore embryos revealed a significant increase in the percentage of DH compared to the control for all substances: ethalfluralin, 62.2%; trifluralin, 58.3%; colchicine, 56.1%; amiprophos-methyl, 54.4%; pronamide, 35.0%; control, 13.3%. The occurrence of pure tetraploids was low (0-5%), mixoploids - especially n+2n - were high in all treatments, including the control (29.4-71.1%). Their presence can be explained as a consequence of spontaneous diploidisation and/or endoreduplication during embryogenesis or embryo germination.

Keywords: endopolyploidy; haploids; in vitro diploidization; microspore embryos; mixoploids (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:60:y:2024:i:2:id:84-2023-cjgpb

DOI: 10.17221/84/2023-CJGPB

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