Transcriptome analyses reveal the toxicity of graphene oxide with different diameters on buckwheat root growth

Changying Liu, Hanlin Wang, Han Li, Qingcheng Qiu, Dabing Xiang and Yanxia Liu
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Changying Liu: Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, Chengdu University, Chengdu, Sichuan, PR China
Hanlin Wang: Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, Chengdu University, Chengdu, Sichuan, PR China
Han Li: Guizhou Academy of Tobacco Science, Guiyang, Guizhou, PR China
Qingcheng Qiu: Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, Chengdu University, Chengdu, Sichuan, PR China
Dabing Xiang: Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering and Technology Research Center of Coarse Cereal Industrialization, Chengdu University, Chengdu, Sichuan, PR China

Czech Journal of Genetics and Plant Breeding, 2024, vol. 60, issue 4, 237-248

Abstract: Graphene-based materials (GBMs) have become potential soil pollutants due to their wide applications in agricultural environments. Although physiological mechanisms of plant responses to GBMs have been previously explored, the underlying molecular mechanisms remain unclear. In this paper, we analysed the physiological and transcriptomic changes of buckwheat (Fagopyrum spp.) roots exposed to 100 mg/L graphene oxide (GO) with different diameter. GO negatively affected root growth and higher diameters of GO caused more adverse effects on the root. In total 3 724 GO-responsive genes were identified in root by transcriptome analysis. 70 differentially expressed genes (DEGs) were involved in ROS detoxification, and 37 transporter-encoding genes were found to be involved in GO response. These transporters may regulate the uptake and transport of GO in buckwheat. The gene expression of 84 transcription factors (TFs) showed a response to GO stress in the root, which may regulate the transporters and reactive oxygen species (ROS) detoxification-related genes. Finally, the difference in the transcriptomic response of the root to the three GO materials with different diameters was investigated. 49 GO-responsive genes may be involved in the difference in the toxicity of GO with different diameters. This study provides new insights into the molecular mechanisms of plant roots to GBMs.

Keywords: buckwheat; graphene oxide; phytotoxicity; transcriptome analysis (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:60:y:2024:i:4:id:69-2024-cjgpb

DOI: 10.17221/69/2024-CJGPB

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