Association ana­lysis of the molecular characteristics and floral traits of Iris × germanica

Feng Tian, Ya Tian, Fang Yu, Jinsen Qian, Feijian Wang, Xue Li, Tongyin Li, Xiaofei Zhang, Dazhuang Huang and Xiaojie Zhao
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Feng Tian: Hebei Agricultural University, Baoding, P.R. China
Ya Tian: Hebei Agricultural University, Baoding, P.R. China
Fang Yu: Hebei Agricultural University, Baoding, P.R. China
Jinsen Qian: Hebei Agricultural University, Baoding, P.R. China
Feijian Wang: Hebei Agricultural University, Baoding, P.R. China
Xue Li: Hebei Agricultural University, Baoding, P.R. China
Tongyin Li: Department of Plant and Soil Sciences, Mississippi State University, Mississippi State, USA
Xiaofei Zhang: Hebei Academy of Forestry and Grassland Science, Shijiazhuang, China
Dazhuang Huang: Hebei Agricultural University, Baoding, P.R. China
Xiaojie Zhao: Hebei Agricultural University, Baoding, P.R. China

Czech Journal of Genetics and Plant Breeding, 2025, vol. 61, issue 2, 55-66

Abstract: Iris × germanica L. (bearded iris) is a popular ornamental plant with numerous commercially important cultivars; however, little is known about the genetic diversity and population structure of the species, as limited DNA markers have been explored. In this study, 34 722 expressed sequence tag (EST)-simple sequence repeat (SSR) loci were identified from RNA sequencing data. The most abundant SSR motifs belonged to the tri-nucleotide type, of which the most common were AGG/CCT followed by AAG/CTT. Overall, 50 primer pairs derived from these EST-SSRs were randomly selected and synthesized, and 22 primer pairs with good polymorphism effects were used for the following experiment. Correlation analysis of nine floral traits showed that most floral traits had significant correlations with each other. Association analysis between SSR molecular markers and nine floral traits showed that 11 EST-SSR markers were associated with 3-6 floral traits. The cluster tree constructed by using the unweighted pair group method demonstrated that the cultivars that had the same parents or similar colour were clustered together. The genotypic relations of most cultivars were consistent with their pedigree-based relationships. The EST-SSR loci identified in this study will facilitate the exploitation of genetic resources and molecular breeding of I. × germanica.

Keywords: bearded iris; EST-SSR markers; flower; genetic diversity; RNA-Seq (search for similar items in EconPapers)
Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:61:y:2025:i:2:id:93-2024-cjgpb

DOI: 10.17221/93/2024-CJGPB

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