Mechanisms of overwintering frost tolerance in alfalfa roots based on 4D-Label-free quantitative proteomics

Yajun Ma, Yu Zhang, Qiaoxian Zhang, Guo Sun, Yajun Yan, Chuan Wang, Wenbin Mian, Tianhui Yang and Ting Gao
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Yajun Ma: Institute of Animal Science (Grass Livestock Engineering and Technology Research Center), Ningxia Academy of Agriculture and Forestry, Yinchuan,Ningxia, P.R. China
Yu Zhang: Ningxia Grassland Workstationr, Ningxia Autonomous Region Forestry and Grassland Bureau, Yinchuan,Ningxia, P.R. China
Qiaoxian Zhang: Ningxia Autonomous Region Animal Husbandry Workstation, Yinchuan, Ningxia, P.R.China
Guo Sun: Yan Chi County Forestry and Grassland Bureau, Yan Chi, Ningxia, P.R. China
Yajun Yan: College of Life Sciences, Sichuan University, Chengdu, Sichuan, P.R. China
Chuan Wang: Institute of Animal Science (Grass Livestock Engineering and Technology Research Center), Ningxia Academy of Agriculture and Forestry, Yinchuan,Ningxia, P.R. China
Wenbin Mian: Institute of Animal Science (Grass Livestock Engineering and Technology Research Center), Ningxia Academy of Agriculture and Forestry, Yinchuan,Ningxia, P.R. China
Tianhui Yang: Institute of Animal Science (Grass Livestock Engineering and Technology Research Center), Ningxia Academy of Agriculture and Forestry, Yinchuan,Ningxia, P.R. China

Czech Journal of Genetics and Plant Breeding, vol. preprint

Abstract: The present study investigates the differential protein expression levels between the frost-tolerant variety Qingda No.1 and the frost-sensitive variety Gannong No.9. The analysis was conducted using 4D-Label-free quantitative proteomics technology, with the samples collected prior to and after overwintering. The results showed that the protein expression of Qingda No.1 changed more significantly during the overwintering process, with 451 differentially expressed proteins (DEPs) being identified, of which 224 were up-regulated and 227 down-regulated. In contrast, the protein expression of Gannong No.9 differed from that of alfalfa roots of the frost-sensitive variety, with 204 DEPs being identified, of which 93 were up-regulated and 111 down-regulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the phenylpropanoid biosynthesis pathway was significantly enriched in both groups. Furthermore, enzymes such as phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone isomerase (CHI) were significantly up-regulated in the freezing-tolerant varieties. Protein interaction network analysis revealed the synergistic regulation mode of DEPs. The present study elucidated the metabolic adaptive mechanism of overwintering in alfalfa roots at the protein level, thus providing a theoretical basis for the selection and breeding of frost-tolerant varieties.

Keywords: differentially expressed protein; enri­chment analysis; low-temperature stress; Medicago sativa; phenylpropanoid biosynthesis (search for similar items in EconPapers)
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjg:v:preprint:id:27-2025-cjgpb

DOI: 10.17221/27/2025-CJGPB

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