The ambiguity of freemartinism diagnosis in cattle revealed by cytogenetic and molecular techniques

J. Nowacka, M. Switonski, M. Mackowski, E. Slota, A. Radko, T. Zabek and K. Urbaniak
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J. Nowacka: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U
M. Switonski: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U
M. Mackowski: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U
E. Slota: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U
A. Radko: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U
T. Zabek: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U
K. Urbaniak: , M. S 1, M. M 1, E. S 2, A. R 2, T. Z 2, K. U

Czech Journal of Animal Science, 2004, vol. 49, issue 6, 239-243

Abstract: Nineteen heifers and three male co-twins, originating from heterosexual twin pregnancies, were analysed with the use of cytogenetic and molecular techniques. A large number (50-400) of Giemsa stained metaphase spreads were studied and the proportion of XX and XY cell lines was calculated. The presence of two genes - SRY and AMEL (AMELX and AMELY) - was also analysed. Both approaches facilitated the identification of the XX/XY chimerism in 17 females and three males. The proportion of the co-twin cell line ranged from 1 to 99%. In two females no chimerism was detected: (1) 60,XY male chromosome complement in all 400 metaphase spreads; SRY-positive, AMELY-positive, AMELX-positive and (2) 60,XX female chromosome complement in all 200 metaphase spreads; SRY-negative, AMELY-negative, AMELX-positive. The usefulness of different techniques for the diagnosis of freemartinism is discussed.

Keywords: intersexuality; freemartinism; XX/XY chimerism; cattle (search for similar items in EconPapers)
Date: 2004
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:49:y:2004:i:6:id:4306-cjas

DOI: 10.17221/4306-CJAS

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