Separation and identification of carp pituitary proteins and glycoproteins

H. Ryšlavá, M. Janatová, G. Čalounová, I. Selicharová, J. Barthová and T. Barth
Additional contact information
H. Ryšlavá: Department of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech Republic
M. Janatová: Department of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech Republic
G. Čalounová: Department of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech Republic
I. Selicharová: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
J. Barthová: Department of Biochemistry, Faculty of Science, Charles University of Prague, Prague, Czech Republic
T. Barth: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Czech Journal of Animal Science, 2005, vol. 50, issue 9, 430-437

Abstract: Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The protein fractions were analysed by SDS-PAGE. Luteinizing hormone (in the α1β and α2β forms), growth hormone, free α1 subunit and β subunit of thyroid-stimulating hormone were identified by N-terminal amino acid sequencing. N-linked oligosaccharide chains of thyroid-stimulating hormone (β TSH) were separated after fluorescent labelling on a GlycoSep N column and treated by exoglycosidases. Among the saccharide components, complex and hybrid structures terminating SO4-GalNAc-GlcNAc-Man-, and high mannose structures, with 1 to 8 mannose units attached to the oligosaccharide core (GlcNAc)2(Man)3, were found.

Keywords: carp hormones; glycoproteins; immobilized metal ion affinity chromatography (IMAC); oligosaccharide chains (search for similar items in EconPapers)
Date: 2005
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/4232-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/4232-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:50:y:2005:i:9:id:4232-cjas

DOI: 10.17221/4232-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().