Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor

J. Petr, E. Chmelíková, A. Dörflerová, M. Ješeta and Z. Kuthanová
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J. Petr: Institute of Animal Science, Prague-Uhříněves, Czech Republic
E. Chmelíková: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic
A. Dörflerová: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic
M. Ješeta: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic
Z. Kuthanová: Department of Veterinary Sciences, Czech University of Life Sciences in Prague, Prague-Suchdol, Czech Republic

Czech Journal of Animal Science, 2007, vol. 52, issue 12, 415-422

Abstract: Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC) inhibitors, bisindolylmaleimide I or rottlerin, are able to inhibit parthenogenetic activation induced by calcium ionophore. The rate of activated oocytes decreased from 69% to 2% (P < 0.05) under the effect of bisindolylmaleimide I at a concentration of 0 or 20nM, respectively. The activation rate decreased from 68% to 0% (P < 0.05) under the influence of 0 or 20μM rottlerin, respectively. PKC inhibitors Go6976 or hispidin had no effect on the oocyte activation using calcium ionophore or on oocytes activated by a nitric oxide donor. The activation of oocytes by a nitric oxide donor is not significantly influenced even under the effects of bisindolylmaleimide I or rottlerin. Based on these data we can conclude that the oocyte activation induced by calcium ionophore depends on PKC, especially on PKC-δ. On the other hand, the oocyte activation induced by nitric oxide is independent of the tested isotypes of PKC.

Keywords: oocyte; pig; parthenogenesis; activation; calcium ionophore; nitric oxide (search for similar items in EconPapers)
Date: 2007
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DOI: 10.17221/2336-CJAS

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