Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens

M. Simon, J. Antalíková, Ľ. Horovská, J. Jankovičová, K. Fábryová, S. Hluchý, P. Chrenek and V. Tančin
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M. Simon: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic
J. Antalíková: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic
Ľ. Horovská: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic
J. Jankovičová: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic
K. Fábryová: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic
S. Hluchý: Slovak University of Agriculture, Nitra, Slovak Republic
P. Chrenek: Slovak University of Agriculture, Nitra, Slovak Republic
V. Tančin: Slovak Agricultural Research Centre, Research Institute of Animal Production, Nitra, Slovak Republic

Czech Journal of Animal Science, 2009, vol. 54, issue 6, 270-276

Abstract: Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules on rabbit cells by mAbs IVA-50 and IVA-38. It showed that IVA-50 is a new valuable CD9 reagent for rabbit immunology which could be used for immunofluorescence staining or ELISA assay, immunohistological and molecular studies of rabbit CD9 antigen. IVA-38 recognizes the CD41/61 on rabbit platelets in indirect immunofluorescence and ELISA assay.

Keywords: blood cells; tissue; CD9; CD41/61 (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:54:y:2009:i:6:id:1728-cjas

DOI: 10.17221/1728-CJAS

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