Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs

Z. Vykoukalová, A. Knoll and S. Čepica
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Z. Vykoukalová: Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry Brno, Brno, Czech Republic
A. Knoll: Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry Brno, Brno, Czech Republic
S. Čepica: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic

Czech Journal of Animal Science, 2009, vol. 54, issue 8, 359-364

Abstract: Perilipin, encoded by the PLIN gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine PLIN gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G>A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan × Piétrain cross. Associations were studied between loci g.4119A>G and g.7966T>C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content (LMC) in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A>G and g.7966T>C only genotype combinations AA-TT, AG-TC and GG-CC were detected. A significant difference (P = 0.0290) between GG-CC and AG-TC genotype combinations for BFT in herd 2 and a suggestive difference (P = 0.0516) between GG-CC and AA-TT genotypes for ADG in herd 1 were detected.

Keywords: pig; perilipin; polymorphism; association analysis (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:54:y:2009:i:8:id:1661-cjas

DOI: 10.17221/1661-CJAS

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