Effects of a probiotic in combination with prebiotics on intestinal lactobacilli and coliforms and activities of bacterial enzymes in 1,2-dimethylhydrazine exposed rats

L. Strojný, A. Bomba, E. Hijová, A. Chmelárová, G. Mojžišová, I. Bertková, J. Koprovičová, M. Pomfy, V. Strompfová and M. Molokáčová
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L. Strojný: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
A. Bomba: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
E. Hijová: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
A. Chmelárová: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
G. Mojžišová: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
I. Bertková: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
J. Koprovičová: Institute of Experimental Medicine, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
M. Pomfy: Institute of Histology and Embryology, Faculty of Medicine, P. J. Šafárik University, Košice, Slovak Republic
V. Strompfová: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovak Republic
M. Molokáčová: Department of Microbiology, Teaching Hospital, Košice, Slovak Republic

Czech Journal of Animal Science, 2011, vol. 56, issue 3, 99-106

Abstract: Effects of the probiotic (PRO) Lactobacillus plantarum and of the combination of PRO and the prebiotic (PRE) inulin enriched with oligofructose (2%), and PRO with Lini oleum virginale (O) on counts of lactobacilli and coliforms and enzymatic activities in faeces of rats were studied. The rats (n = 60) were divided into 5 groups of 12 subjects. The animals were fed on a high fat diet (10%) for 8 weeks of experiment. Colon cancer was induced by the application of 1,2-dimethylhydrazine (DMH) twice a week in a dose of 20 mg/kg s.c. in groups G2-G5. The rats in group 1 (control 1) received a diet without any supplements. The rats in group 2 (control 2) received 1,2 DMH without any supplements. The rats in group 3 received PRO, group 4 PRO and PRE, and group 5 received PRO and O. A significant decrease (P < 0.05) of coliforms was found out after the application of PRO, PRO-O, and PRO-PRE in comparison with control group G2. Significantly higher (P < 0.05) counts of lactobacilli were determined after the application of PRO-O and PRO-PRE. Significantly lower (P < 0.001) activities of β-galactosidase, β-glucuronidase and α-glucosidase were observed in PRO, PRO-PRE and PRO-O, while in the case of the enzyme β-glucosidase the activity was lower only after the addition of PRO-O. The protective effect of lactobacilli was observed in the order PRO-O, PRO-PRE, and PRO. It was shown that combinations of PRO-O and PRO-PRE had a synergistic effect which was higher than the effect of administering only PRO.

Keywords: L. plantarum; inulin; 1; 2-dimethylhydrazine; lactobacilli; coliforms (search for similar items in EconPapers)
Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:56:y:2011:i:3:id:56-2010-cjas

DOI: 10.17221/56/2010-CJAS

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