Rapid sexing of selected Galliformes by polymerase chain reaction

J. Kalina, J. Mucksová, H. Yan and P. Trefil
Additional contact information
J. Kalina: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s., Jílové u Prahy, Czech Republic
J. Mucksová: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s., Jílové u Prahy, Czech Republic
H. Yan: Hunan Institute of Animal and Veterinary Science, Hunan, P.R. China
P. Trefil: BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s., Jílové u Prahy, Czech Republic

Czech Journal of Animal Science, 2012, vol. 57, issue 4, 187-192

Abstract: Vent sexing of one-day-old chicks in commercial hatcheries has long been common practice and can be highly accurate. However, there are circumstances when this technique is not applicable such as smaller breeds, non-domestic birds, or where is the necessity of precise sexing. In this study we present a simple and reliable method for fast gender determination in selected Galliformes for which phenotypic determination of sex is difficult until maturity. Four species were tested: two commercial species - chicken (Gallus gallus) and turkey (Meleagris gallopavo), and two game birds - common pheasant (Phasianus colchicus) and wood grouse (Tetraro urogallus). DNA was tested with universal single-pair primers polymerase chain reaction (PCR) detecting W chromosome specific sequence yielding a single band of length specific for each species. The method was developed with regards to time consumption and cost-effectiveness giving results in less than two hours. The method may also be used for early sexing in commercial chicken and turkey flocks as well as sexing of smaller game birds flocks or for research laboratories when rapid sexing of selected Galliformes cells is required.

Keywords: sex determination; fowl; game fowl; polymerase chain reaction (search for similar items in EconPapers)
Date: 2012
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/5894-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/5894-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:57:y:2012:i:4:id:5894-cjas

DOI: 10.17221/5894-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().