Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

M. Ziaullah, A. Ijaz, M. Aleem, A.K. Mahmood, H. Rehman, S. Bhatti, U. Farooq and M.U. Sohail
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M. Ziaullah: Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan
A. Ijaz: Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
M. Aleem: Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, Pakistan
A.K. Mahmood: Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan
H. Rehman: Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan
S. Bhatti: Pet Centre, University of Veterinary and Animal Sciences, Lahore, Pakistan
U. Farooq: University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan
M.U. Sohail: Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

Czech Journal of Animal Science, 2012, vol. 57, issue 8, 377-381

Abstract: The study was conducted to evaluate the potential cryoprotective effect of butylated hydroxytoluene (BHT) through post-thaw evaluation of canine semen and its optimal inclusion level. Ejaculated canine semen was extended in TRIS-glucose egg yolk extender containing various concentrations of BHT (0.5, 1.0, 1.5, 2.0, and 2.5mM). Semen was frozen at -196°C using 200 × 106 spermatozoa per 0.5 ml straws and post-thaw evaluation was carried out in terms of sperm motility, viability, plasma membrane integrity, and acrosomal integrity through phase-contrast microscope, supravital staining, hypo-osmotic swelling test, and normal acrosomal ridge, respectively. BHT was found to improve (P > 0.005) all post-thawed semen quality parameters at an inclusion level of 1.0mM in the extended semen. However, higher concentrations than this were found to have detrimental effects.

Keywords: canine; butylated hydroxytoluene; cryopreservation; sperm quality (search for similar items in EconPapers)
Date: 2012
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:57:y:2012:i:8:id:6273-cjas

DOI: 10.17221/6273-CJAS

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