Retinoic acid attenuates oxidative injury in bovine mammary epithelial cells induced by hydrogen peroxide

Lu Jin, Sumei Yan, Binlin Shi, Huiyu Shi, Xiaoyu Guo and Junliang Li
Additional contact information
Lu Jin: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
Binlin Shi: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
Huiyu Shi: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
Xiaoyu Guo: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China
Junliang Li: College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P.R. China

Czech Journal of Animal Science, 2017, vol. 62, issue 12, 539-548

Abstract: The objective of this study was to explore how retinoic acid (RA) attenuates oxidative injury induced by hydrogen peroxide (H2O2) in bovine mammary epithelial cells (BMEC). Subconfluence BMEC were randomly divided into four groups with six replicates: the control group (incubated in serum-free medium without RA or H2O2 for 30 h), H2O2 group (pre-incubated for 24 h without RA, then for another 6 h with 600 μM H2O2), RA group (incubated with 1 mg/ml RA for 30 h without H2O2), and RA + H2O2 group (RA prevention group, pre-incubated with 1 mg/ml RA for 24 h and then for another 6 h with 600 mM H2O2). The results showed that the H2O2 treatment significantly decreased several measured traits, including the cell viability, glutathione peroxidase (GPX) and thioredoxin reductase (TRXR) activities, selenoprotein P (SELP) content, catalase and superoxide dismutase activities, total antioxidant capacity, and GPX1, TRXR1, and SELP gene expression, as well as GPX1 and TRXR1 protein expression. H2O2 treatment also increased the malondialdehyde and reactive oxygen species contents and induced a marked increase of several measured traits, including the arachidonic acid (ARA) concentration, cytosolic phospholipase A2 and 5-lipoxygenase gene expression and activity, and 15-hydroxy twenty-four arachidonic acid and hydroxy peroxide tetracosenic arachidonic acid contents. RA pre-treatment prevented corresponding increases in parameters related to ARA metabolism and increased the activity of TRXR. Moreover, RA pre-treatment attenuated the phosphorylation levels of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase and effectively decreased the ARA content. These results suggest that RA protected BMEC from oxidative stress by elevating TRXR activity, which inhibited the MAPK signaling pathway and led to a decreased concentration of ARA.

Keywords: vitamin A; thioredoxin reductase; oxidative injury; arachidonic acid; p38 mitogen-activated protein kinase (search for similar items in EconPapers)
Date: 2017
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/42/2016-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/42/2016-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:62:y:2017:i:12:id:42-2016-cjas

DOI: 10.17221/42/2016-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().