Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickens

Yi Wan, Chendong Ma, Peipei Wei, Qi Fang, Xing Guo, Bangyuan Zhou and Runshen Jiang
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Yi Wan: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
Chendong Ma: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
Peipei Wei: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
Qi Fang: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
Xing Guo: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China
Bangyuan Zhou: College of Animal Science and Technology, Anhui Agricultural University, Hefei, P.R. China

Czech Journal of Animal Science, 2017, vol. 62, issue 2, 82-87

Abstract: The effects of heat stress on HSP90B1 messenger RNA (mRNA) expression in the hypothalamus of chicken were investigated and HSP90B1 variations were detected. Females of two Chinese chicken breeds (Huainan and Wenchang) were used for the experiments. At 64 days of age, the ambient temperature (24 ± 1°C) was increased to 35 ± 1°C for 24 h (heat stress), then decreased to 24 ± 1°C for 24 h (recovery). Hypothalamus samples were collected at 0, 12, and 24 h during heat stress, as well as 12 and 24 h during recovery. The HSP90B1 mRNA expression increased significantly during heat stress and significantly decreased during recovery being higher in Huainan chickens. Fifteen primer pairs were designed to amplify the exons of HSP90B1 by a polymerase chain reaction, and single nucleotide polymorphisms (SNPs) were detected by Sanger sequencing. In Huainan chickens, we identified a SNP (NC_006088.3:g.6798G>A) in exon 14 of HSP90B1 which did not cause amino acid variation but caused a codon for glutamic acid change from GAG to GAA. The frequencies for genotypes AA, GA, and GG were 0.49, 0.27, and 0.24, respectively. Individuals with the GG genotype survived heat stress at 42°C for a longer time (248.2 ± 39.3 min) than individuals with GA and AA genotypes, which survived for 227.2 ± 44.5 min and 179.3 ± 36.5 min, respectively. The results suggested that the increased heat tolerance was associated with the higher expression of HSP90B1, and genotype GG could be used as a potential marker for heat resistance in chickens.

Keywords: heat shock protein; mRNA level; single nucleotide polymorphism; thermal stress (search for similar items in EconPapers)
Date: 2017
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:62:y:2017:i:2:id:8-2016-cjas

DOI: 10.17221/8/2016-CJAS

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