In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation

Susanne Sinz, Carmen Kunz, Annette Liesegang, Ueli Braun, Svenja Marquardt, Carla R. Soliva and Michael Kreuzer
Additional contact information
Susanne Sinz: ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland
Carmen Kunz: ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland
Annette Liesegang: Institute of Animal Nutrition, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
Ueli Braun: Department of Farm Animals, Vetsuisse Faculty, University of Zürich, Zürich, Switzerland
Svenja Marquardt: ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland
Carla R. Soliva: ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland
Michael Kreuzer: ETH Zürich, Institute of Agricultural Sciences, Zürich, Switzerland

Czech Journal of Animal Science, 2018, vol. 63, issue 8, 293-304

Abstract: Polyphenols, like flavonoids, have been investigated when present in intact plants or in extracts as methane mitigating dietary supplements in ruminants. The aim of the present study was to examine pure compounds in a short-term in vitro experiment using the Hohenheim Gas Test method. We focused on the group of the flavonoids and tested which of them had the potential to mitigate methane without negatively affecting ruminal fermentation. Eight flavonoids were tested: epicatechin, luteolin-7-glucoside, quercetin, and isoquercetin in Experiment 1; catechin, gallocatechin, epigallocatechin, and epigallocatechin gallate in Experiment 2. Tannic acid, no flavonoid but a phenolic acid with known methane mitigating properties, served as positive control, and the unsupplemented basal diet as negative control. In both experiments, each of these compounds (including tannic acid) was tested at dosages of 0.5, 5.0, and 50.0 mg/g basal diet dry matter (DM) in four runs each. Gallocatechin, tannic acid, and epigallocatechin gallate (50 mg/g DM) lowered fermentation gas formation and in vitro organic matter digestibility relative to the negative control (Experiment 2). Apart from tannic acid, epicatechin, quercetin, isoquercetin, and luteolin-7-glucoside (5 and 50 mg/g DM) reduced the amount of CH4 produced in relation to total gas produced (Experiment 1). The incubation fluid ammonia concentration was decreased with luteolin-7-glucoside and tannic acid (50 mg/g DM). From the flavonoids tested especially luteolin-7-glucoside seems to have a similar potential as tannic acid to mitigate methane and ammonia formation during ruminal fermentation in vitro, both favourable in environmental respect. These results need to be confirmed in live animals.

Keywords: rumen; ammonia; Hohenheim Gas Test; epicatechin; isoquercetin; luteolin-7-glucoside; quercetin; catechin; tannic acid (search for similar items in EconPapers)
Date: 2018
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/118/2017-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/118/2017-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:63:y:2018:i:8:id:118-2017-cjas

DOI: 10.17221/118/2017-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().