Genomic structure and expression of the porcine ACTC1 gene

Antonín Stratil, Pavel Horák, Michaela Nesvadbová, Mario Van Poucke, Věra Dvořáková, Roman Stupka, Jaroslav Čítek, Kateřina Zadinová, Luc J. Peelman and Aleš Knoll
Additional contact information
Antonín Stratil: Institute of Animal Physiology and Genetics CAS, v.v.i. Liběchov, Liběchov, Czech Republic
Pavel Horák: Institute of Animal Physiology and Genetics CAS, v.v.i. Liběchov, Liběchov, Czech Republic
Michaela Nesvadbová: Department of Morphology, Physiology and Animal Genetics, Mendel University in Brno, Brno, Czech Republic
Mario Van Poucke: Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
Věra Dvořáková: Institute of Animal Physiology and Genetics CAS, v.v.i. Liběchov, Liběchov, Czech Republic
Roman Stupka: Department of Animal Husbandry, Czech University of Life Sciences Prague, Prague, Czech Republic
Jaroslav Čítek: Department of Animal Husbandry, Czech University of Life Sciences Prague, Prague, Czech Republic
Kateřina Zadinová: Department of Animal Husbandry, Czech University of Life Sciences Prague, Prague, Czech Republic
Luc J. Peelman: Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
Aleš Knoll: Department of Morphology, Physiology and Animal Genetics, Mendel University in Brno, Brno, Czech Republic

Czech Journal of Animal Science, 2018, vol. 63, issue 9, 371-378

Abstract: A partial cDNA (~1200 bp) of the porcine ACTC1 gene was identified in the subtracted foetal hind limb muscle cDNA library (44 days of gestation; using m. biceps femoris cDNA as the driver). Using specific polymerase chain reaction (PCR) primers, a bacterial artificial chromosome (BAC) clone containing the genomic ACTC1 gene was identified and the gene was sequenced. Specific PCR primers designed from the BAC and cDNA sequences were used for amplification and comparative sequencing of ACTC1 of Pietrain and Meishan pigs. The gene is approximately 5.4 kb in length, is composed of 7 exons, and has a coding sequence containing 1134 bp. The gene was mapped using the INRA-Minnesota porcine radiation hybrid (IMpRH) panel to chromosome 1, with SW65 as the closest marker (41 cR; LOD = 7.73). Differences were observed in tissue-specific expression of ACTC1 that was studied by transcription profiling in 28 porcine tissues. Developmental differences in muscle and heart were analysed by real-time quantitative PCR (RT-qPCR). Two single nucleotide polymorphisms (SNPs) were found in intron 1. One adequately informative SNP (FM212567.1:g.901C>G) was genotyped by PCR-restriction fragment length polymorphism, and allele frequencies in eight pig breeds were calculated.

Keywords: actin; alpha; cardiac muscle 1; mRNA; pig; transcription profiling; RT-qPCR (search for similar items in EconPapers)
Date: 2018
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/34/2018-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/34/2018-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:63:y:2018:i:9:id:34-2018-cjas

DOI: 10.17221/34/2018-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().