Expression analysis of BMPR1B, BMP15, and GDF9 in prolific and non-prolific sheep breeds during the follicular phase

Ji-Shun Tang, Wen-Ping Hu, Ran Di, Xiang-Yu Wang, Xiao-Sheng Zhang, Jin-Long Zhang, Qiu-Yue Liu and Ming-Xing Chu
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Ji-Shun Tang: Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
Wen-Ping Hu: Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
Ran Di: Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
Xiang-Yu Wang: Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
Xiao-Sheng Zhang: Tianjin Institute of Animal Sciences, Tianjin, P.R. China
Jin-Long Zhang: Tianjin Institute of Animal Sciences, Tianjin, P.R. China
Qiu-Yue Liu: Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China
Ming-Xing Chu: Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China

Czech Journal of Animal Science, 2019, vol. 64, issue 11, 439-447

Abstract: To elucidate how expression characteristics of BMPR1B, BMP15, and GDF9 are associated with sheep reproduction, tissue-specific mRNA expression of these genes in Small Tail Han (STH) ewes (a polytocous breed) and Sunite (SNT) ewes (a monotocous breed) in the follicular phase were investigated using transcription profiling and quantitative real-time PCR (qPCR). Expression levels of the three genes were all highest in ovaries of the two sheep breeds, and BMPR1B and GDF9 expression in the ovarian tissue was significantly higher in STH sheep compared with SNT sheep (P < 0.01), whereas BMP15 expression in pituitary, ovarian, oviduct, and uterine tissues was significantly lower in STH sheep compared with SNT sheep (P < 0.01). This study revealed that BMPR1B, BMP15, and GDF9 may play important roles in the hypothalamic-pituitary-gonadal axis, especially in the ovary, which may affect sheep prolificacy. BMPR1B and GDF9 expression might increase litter size, whereas BMP15 expression may decrease litter size. These results provide preliminary information regarding molecular regulatory mechanisms underlying sheep prolificacy.

Keywords: tissue; candidate gene; transcription profiling; qPCR; prolificacy (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:64:y:2019:i:11:id:101-2018-cjas

DOI: 10.17221/101/2018-CJAS

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