Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro

Francesco Vizzarri, Marisa Palazzo, Donato Casamassima, Lubomir Ondruska, Martin Massanyi, Filip Tirpak, Grzegorz Formicki, Agnieszka Gren and Peter Massanyi
Additional contact information
Francesco Vizzarri: Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy
Marisa Palazzo: Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy
Donato Casamassima: Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy
Lubomir Ondruska: National Agricultural and Food Centre, Nitra, Slovak Republic
Martin Massanyi: Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic
Filip Tirpak: Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic
Grzegorz Formicki: Institute of Biology, Pedagogical University, Krakow, Poland
Agnieszka Gren: Institute of Biology, Pedagogical University, Krakow, Poland
Peter Massanyi: Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak Republic

Czech Journal of Animal Science, 2019, vol. 64, issue 1, 1-10

Abstract: Verbascoside, the main component of Lippia citriodora extract, is one of the most powerful free radical scavengers exhibiting a wide biological activity. In in vivo study 20 adult New Zealand white rabbit bucks were divided into two homogeneous groups, one control (CON) and one verbascoside-supplemented (0.1%) in feed mixture (EXP) and later in vitro effects of verbascoside on the motility aspects of rabbit spermatozoa were analysed. The spermatozoa concentration, ejaculate volume, spermatozoa motility, progressive motility, distance parameters, velocity parameters and type of spermatozoa movement were negatively affected by Lippia citriodora leaves extract after the first 4 weeks of dietary treatment, till the end of experiment (8 weeks). Four weeks after the suspension of feed additive supplementation, all spermatozoa traits values returned to the normality, and in line with CON group. For in vitro findings, ejaculates from 10 male New Zealand white bucks were collected using an artificial vagina. Then it was diluted in physiological saline solution containing different concentrations of verbascoside at the concentration of 0, 0.0024, 0.0219, 0.157, 120.0 mg/ml (Ctrl, VB1, VB2, VB3, VB4 groups, respectively), using a dilution ratio of 1 : 4. The obtained data proved that verbascoside at the concentration of 0.0024 and 0.0219 mg/ml had no adverse effect on spermatozoa. Additionally, we found that verbascoside at higher concentrations (0.157 and 120.0 mg/ml) significantly altered all the motility parameters analysed in the experiment. In conclusion a possible negative effect of verbascoside supplementation into feed mixture (0.1%) on semen quality parameters in rabbit bucks as well as in vitro can be stated, obviously considering that target organs of antioxidant activities of phenylpropanoid glycosides are various. In addition it has to be emphasized that the extract showed a reversible action, since the semen traits of treated animals returned to the normality after the dietary administration period.

Keywords: feed additive; Verbenaceae; rabbit; spermatozoa; motility (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/35/2018-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/35/2018-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:64:y:2019:i:1:id:35-2018-cjas

DOI: 10.17221/35/2018-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().