Expression patterns of GHRL, GHSR, LEP, LEPR, SST and CCK genes in the gastrointestinal tissues of Tibetan and Yorkshire pigs

Wen-Kui Sun, Chi Cheng, Rui Liu, Yi-Hui Chen, Kai Zeng, Xiao-Hui Chen, Yi-Ren Gu, Jiang-Ling Li, Xue-Bin Lv and Rong Gao
Additional contact information
Wen-Kui Sun: Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China
Chi Cheng: College of Chemistry and Life Science, Chengdu Normal University, Chengdu, P.R. China
Rui Liu: Sichuan Academy of Animal Science, Chengdu, P.R. China
Yi-Hui Chen: Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China
Kai Zeng: Sichuan Academy of Animal Science, Chengdu, P.R. China
Xiao-Hui Chen: Sichuan Academy of Animal Science, Chengdu, P.R. China
Yi-Ren Gu: Sichuan Academy of Animal Science, Chengdu, P.R. China
Jiang-Ling Li: Sichuan Academy of Animal Science, Chengdu, P.R. China
Xue-Bin Lv: Sichuan Academy of Animal Science, Chengdu, P.R. China
Rong Gao: Key Laboratory for Bio-Resource and Eco-Environment of Education Ministry, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P.R. China

Czech Journal of Animal Science, 2019, vol. 64, issue 6, 239-247

Abstract: The aim was to characterize the expression patterns of several genes in the gastrointestinal tracts of Tibetan pigs (TP) and Yorkshire pigs (YP) and to explore their correlation with digestion and growth difference of the two breeds. The body weights and growth of YP and TP were studied at 6, 12 and 24 weeks of age, and their plasma levels of ghrelin (GHRL), leptin (LEP), somatostatin (SST) and cholecystokinin (CCK) were determined by enzyme linked immunosorbent assay (ELISA). Blood and gastrointestinal sections (stomach, duodenum, jejunum, ileum, caecum and colon) were collected and assayed for mRNA expression of the six genes (GHRL, ghrelin receptor (GHSR), LEP, leptin receptor (LEPR), SST and CCK) by reverse transcription-qPCR (RT-qPCR). TP generally had higher mRNA expressions of GHSR, LEP, LEPR, SST and CCK genes compared to YP, and expressed lower levels of the GHRL gene in most tissues of the digestive tract. In both breeds, plasma levels of the expressed proteins were more closely correlated with the feed intake and growth than with mRNA levels of the target genes. Our data indicate that TP possess special gene expression patterns in the gastrointestinal tract compared to YP, which is consistent with its unique feed intake and adaptation to harsh environment.

Keywords: gene transcript; hormone level; growth performance; digestive traits (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/40/2018-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/40/2018-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:64:y:2019:i:6:id:40-2018-cjas

DOI: 10.17221/40/2018-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().