Effect of semen extender supplementation with sericin on post-thaw dairy bull sperm quality and lipid peroxidation

Yotsapon Yangngam, Suttiwat Chapanya, Thevin Vongpralub, Wuttigrai Boonkum and Vibuntita Chankitisakul
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Yotsapon Yangngam: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
Suttiwat Chapanya: Lumphayaklang Livestock Semen Production Center, Lopburi, Thailand
Thevin Vongpralub: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
Wuttigrai Boonkum: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
Vibuntita Chankitisakul: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand

Czech Journal of Animal Science, 2021, vol. 66, issue 1, 13-20

Abstract: Silk sericin plays a protective role in a variety of mammalian cells during cryopreservation. This study aimed to determine the effects of sericin supplementation to a freezing extender on sperm quality and lipid peroxidation in dairy bull sperm cryopreservation. Each semen sample of five Holstein-Friesian crossbred bulls was divided into four aliquots and diluted in a tris-egg yolk extender supplemented with different concentrations of sericin [0%, 0.25%, 0.5%, and 1.0% (wt/vol)]. Sperm motility (CASA), viability, acrosome integrity, mitochondrial membrane potential (fluorescent staining) and lipid peroxidation (malondialdehyde - MDA test) were analysed. The results show that the 0.25% and 0.5% sericin groups had the highest total sperm motility (P < 0.05). Sperm viability, acrosome integrity, and mitochondrial function were higher in the group supplemented with 0.25% sericin compared to the control and 1.0% (P < 0.05). Sericin supplementation with 0.25% and 0.5% significantly decreased MDA concentrations compared with the control (P < 0.05). In conclusion, supplementation of the semen freezing extender with sericin at the concentration of 0.25% significantly improved the post-thaw semen quality and reduced lipid peroxidation in Holstein-Friesian crossbred bulls.

Keywords: antioxidant; cattle; frozen semen; ROS; semen cryopreservation (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:66:y:2021:i:1:id:104-2020-cjas

DOI: 10.17221/104/2020-CJAS

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