Ubiquitin-specific peptidase 8 regulates proliferation and early differentiation of sheep skeletal muscle satellite cells

Shaoyu Wang, Kui Li, Hui Gao, Zepeng Liu, Shuang Shi, Qiang Tan and Zhengguang Wang
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Shaoyu Wang: College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China
Kui Li: Zhejiang Animal Husbandry Techniques Extension Station, Hangzhou, P.R. China
Hui Gao: Zhejiang Animal Husbandry Techniques Extension Station, Hangzhou, P.R. China
Zepeng Liu: College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China
Shuang Shi: College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China
Qiang Tan: College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China
Zhengguang Wang: College of Animal Sciences, Zhejiang University, Hangzhou, P.R. China

Czech Journal of Animal Science, 2021, vol. 66, issue 3, 87-96

Abstract: Ubiquitin-specific protease 8 (USP8), a member of the ubiquitin-specific protease (USP) family, was originally identified as playing a role in the regulation of growth and cell cycle. However, its functional role in myogenesis is unknown. In this study, we investigated the role of USP8 in proliferation and differentiation of sheep skeletal muscle satellite cells. The results showed that the expression level of USP8 was significantly increased on days 2 and 3 following the induction of the differentiation process. Furthermore, knocking down USP8 resulted in a significant increase in myogenin-positive cells, and promoted early differentiation of satellite cells by regulating the expression level of paired box 7 (PAX7). Additionally, knocking down USP8 suppressed muscle satellite cell proliferation, possibly explaining that the relative mRNA level of USP8 was linearly related to muscle fibre density of Hu sheep. Overall, our research demonstrates that USP8 plays a role in proliferation and early differentiation of skeletal muscle satellite cells.

Keywords: myogenesis; myogenin; desmin; myosin heavy chain 3; PAX7 (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:66:y:2021:i:3:id:105-2020-cjas

DOI: 10.17221/105/2020-CJAS

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