Expression of microRNAs in the hypothalamus of pregnant and non-pregnant goats

Lu Zhu, Jingtong Huang, Jing Jing, Qi Zheng, Qianyun Ji, Ya Liu, Jianping Ding, Fugui Fang, Yunsheng Li, Yunhai Zhang and Yinghui Ling
Additional contact information
Lu Zhu: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Jingtong Huang: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Jing Jing: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Qi Zheng: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Qianyun Ji: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Ya Liu: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Jianping Ding: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Fugui Fang: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Yunsheng Li: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Yunhai Zhang: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China
Yinghui Ling: College of Animal Science and Technology, Anhui Agricultural University, Anhui Hefei, P.R. China

Czech Journal of Animal Science, 2021, vol. 66, issue 5, 156-167

Abstract: MicroRNAs (miRNAs) play a significant role in animal reproduction by regulating the expression of protein-coding genes. The hypothalamus regulates the pregnancy cycle changes in goats; however, the action mechanism of miRNAs in this regulation remains to be investigated. In this study, we performed RNA sequencing of hypothalamus samples to establish a comprehensive miRNA profiling of pregnant and non-pregnant goats. A total of 384 miRNAs were identified in the hypothalamus of pregnant goats, of which 239 were newly discovered, and 390 miRNAs were detected in the hypothalamus of non-pregnant goats of which 192 were novel miRNAs. In addition, a total of 280 differentially expressed miRNAs are characterized, of which 171 were known miRNAs and 109 were novel miRNAs. Functional enrichment suggests that the predicted target genes of differentially expressed miRNAs may be involved in the reproductive process. This preliminary study revealed that let-7f-5p, miR-99a-5p and miR-100-5p may be involved in the hypothalamic regulation of pregnancy cycle changes in goats. These data provide a basic reference for subsequent studies on the regulatory role of miRNAs in mammalian pregnancy.

Keywords: miRNA; RNA-seq; reproduction; differential expression (search for similar items in EconPapers)
Date: 2021
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/113/2020-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/113/2020-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:66:y:2021:i:5:id:113-2020-cjas

DOI: 10.17221/113/2020-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().