Dietary supplementation of Lactobacillus zeae regulated the gut microbiome in piglets infected with enterotoxigenic Escherichia coli

Qian Zhang, Lijuan Zhang, Yang Lyu, Yutao Shi, Liangyun Zhu, Min Zhang, Yuyan Zhao, Di Zhao, Lei Wang, Dan Yi, Yongqing Hou and Tao Wu
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Qian Zhang: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Lijuan Zhang: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Yang Lyu: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Yutao Shi: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Liangyun Zhu: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Min Zhang: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Yuyan Zhao: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Di Zhao: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Lei Wang: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Dan Yi: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China
Yongqing Hou: Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University (WPHU), Wuhan, P.R. China

Czech Journal of Animal Science, 2022, vol. 67, issue 1, 27-38

Abstract: This study was conducted to investigate the effect of Lactobacillus zeae LB2 on the gut microbiota in piglets infected with enterotoxigenic Escherichia coli (ETEC). Thirty-two healthy 7-day-old piglets were randomly divided into four treatment groups: control group (basal diet), LB2 group (supplemented with 1 × 108 CFU/pig/day L. zeae LB2), ETEC group (infected with 1 × 1010 CFU/pig/day ETEC) and ETEC+LB2 group (LB2 supplementation + ETEC infection). Intestinal contents were collected for DNA extraction and Illumina sequencing. Significant result was observed for alpha diversity in the four intestinal sections, and both ETEC infection and LB2 supplementation showed a higher Chao1 alpha diversity. At the phylum level, Firmicutes and Bacteroidetes were dominant in the healthy piglets, while Proteobacteria were dominant in the ETEC-infected piglets. At the genus level, ETEC infection decreased the abundance of Prevotella, Ruminococcaceae, Lactobacillus, Alloprevotella, Flavobacterium, and Sutterella and increased the abundance of Actinobacillus. The LB2 supplementation reduced the abundance of Ruminococcaceae, Actinobacillus, Porphyromonas, and Alloprevotella, and increased the abundance of Prevotella and Lactobacillus. Both ETEC infection and LB2 supplementation affected several functional pathways associated with cellular processes, environmental information processing, genetic information processing, diseases, metabolism, and organismal systems. In summary, ETEC infection induced dysbiosis of the gut microbiome in piglets, while L. zeae supplementation could positively regulate the gut microbiome during ETEC infection. Therefore, L. zeae LB2 may be an ideal probiotic for the prevention or treatment of ETEC infection.

Keywords: alpha diversity; ETEC; gut microbiota; L. zeae; pig (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:67:y:2022:i:1:id:136-2021-cjas

DOI: 10.17221/136/2021-CJAS

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