Comparative study: Efficacy of egg-yolk vs soy lecithin-based diluent in preservation of chilled bovine semen - Bacteriology and sperm quality

Michal Ďuračka, Filip Benko, Miroslava Kačániová and Eva Tvrdá
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Michal Ďuračka: AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Filip Benko: Institute of Biotechnology, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Miroslava Kačániová: Institute of Horticulture, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Eva Tvrdá: Institute of Biotechnology, Slovak University of Agriculture in Nitra, Nitra, Slovakia

Czech Journal of Animal Science, 2024, vol. 69, issue 10, 400-409

Abstract: This study compared the efficacy of the egg yolk (EY) and soy lecithin (SL) semen extender during 72-h storage at 4 °C in the presence/absence of streptomycin, penicillin, lincomycin, and spectinomycin. Bull semen samples (n = 12) were obtained and stored in either ANDROMED® (SL extender) or TRILADYL CSS® (EY extender). After 24, 48, and 72 h of storage at 4 °C, bacterial colonies (CFU - colony forming units) were grown and counted on tryptic soy and blood agar, and the bacterial isolates were identified using the MALDI-TOF MS Biotyper. Sperm motility, mitochondrial membrane potential (MMP), membrane and acrosome integrity, sperm DNA fragmentation, and reactive oxygen species (ROS) production were analysed. The results showed that the type of semen diluent could be crucial for the efficacy of used antibiotics, since significantly decreased bacterial occurrence following 48 h or no bacterial growth after 72 h was recorded in the SL-based groups. Even the SL medium itself did not favour bacterial growth, as significantly decreased bacterial load was observed after 48 h (P < 0.01) and 72 h (P <0.000 1). In contrast, the bacterial load in the EY medium without antibiotics significantly increased (P < 0.05). In contrast, an improved ability to preserve sperm quality parameters was observed in EY-extended semen. In particular, sperm motility was significantly higher (P < 0.000 1 and P < 0.001) after 24 h in each EY-based group in comparison to the control (Ctrl), whereas among the SL-based groups, only the spectinomycin-supplemented group presented with a significant motility improvement (P < 0.01) when compared to the Ctrl. Although the EY semen extender provided enhanced sperm quality preservation during the 72-h storage, in the future, the SL medium composition should be improved to match the sperm preservation ability of the EY medium while maintaining its microbial safety.

Keywords: bacteria; bull semen extender; cooled spermatozoa; liquid semen storage (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:69:y:2024:i:10:id:138-2024-cjas

DOI: 10.17221/138/2024-CJAS

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