Quality and in vitro fertilising ability of cryostored Pinzgau bull insemination doses

Lucia Olexiková, Linda Dujíčková, Jaromír Vašíček, Andrej Baláži, Andrea Svoradová, Lenka Kuželová, Alexander Makarevich, Peter Ivanič and Peter Chrenek
Additional contact information
Lucia Olexiková: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Linda Dujíčková: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Jaromír Vašíček: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Andrej Baláži: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Andrea Svoradová: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Lenka Kuželová: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Alexander Makarevich: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Peter Ivanič: Slovak Biological Services, Nitra, Slovak Republic
Peter Chrenek: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic

Czech Journal of Animal Science, 2024, vol. 69, issue 11, 431-440

Abstract: Applicability of cryopreserved insemination doses is dependent on the quality of the semen after thawing. The aim of the study was to check the options of new markers for the assessment of sperm quality to predict their fertilisation ability. In five bulls of the Pinzgau breed, the sperm fertilising ability was determined using the in vitro fertilisation. Bulls were divided according to the achieved blastocyst rate: bulls with good fertilising ability (NOB - 38.6%, KAZ - 28.9%, GAL - 29.3%) and bulls with low fertilising ability (LOH - 19.4%, NUS - 22.1%). In addition to the sperm motility and morphology, we monitored common physiological sperm characteristics: viability, apoptosis, acrosomal status, capacitation, mitochondrial activity and generation of reactive oxygen species using the flow cytometry procedure. Novel fertility-related biomarkers, such as ubiquitination, overexpression of MKRN1, SPTRX-3 and PAWP proteins or histone modification (H3K4me2), were also analysed by flow cytometry. From all monitored parameters, more proper characteristics of impaired in vitro fertilising ability proved to be high incidence of apoptotic markers (YO-PRO, Caspase 3/7) and higher counts of morphologically abnormal spermatozoa. Although the in vitro fertilisation (IVF) test can be an advantageous method for evaluating the sperm fertilising ability, there are still differences between the in vivo and in vitro fertilisation processes, which must be considered for example, when evaluating the sperm capacitation status.

Keywords: biomarker; bovine; fertilisation; flow cytometry; sperm (search for similar items in EconPapers)
Date: 2024
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://cjas.agriculturejournals.cz/doi/10.17221/142/2024-CJAS.html (text/html)
http://cjas.agriculturejournals.cz/doi/10.17221/142/2024-CJAS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:69:y:2024:i:11:id:142-2024-cjas

DOI: 10.17221/142/2024-CJAS

Access Statistics for this article

Czech Journal of Animal Science is currently edited by Bc. Michaela Polcarová

More articles in Czech Journal of Animal Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().