The effect of ellagic acid on rabbit sperm in vitro parameters after cryopreservation

Jovana Grba, Lenka Kuželová, Alexander Makarevich, Andrej Baláži, Saša Dragin, Dragana Tekić and Peter Chrenek
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Jovana Grba: University of Novi Sad, Faculty of Agriculture, Department of Animal Science, Serbia
Lenka Kuželová: Faculty of Biotechnology and Food Science, Slovak University of Agriculture, Nitra, Slovak Republic
Alexander Makarevich: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Andrej Baláži: Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Lužianky, Slovak Republic
Saša Dragin: University of Novi Sad, Faculty of Agriculture, Department of Animal Science, Serbia
Dragana Tekić: University of Novi Sad, Faculty of Agriculture, Department of Agroeconomy, Serbia
Peter Chrenek: Faculty of Biotechnology and Food Science, Slovak University of Agriculture, Nitra, Slovak Republic

Czech Journal of Animal Science, 2024, vol. 69, issue 3, 110-117

Abstract: Cryopreservation reduces the antioxidant activity of spermatozoa and makes them more sensitive to damage caused by reactive oxygen species (ROS). The addition of antioxidants to the freezing medium could prevent cryo-damage by mitigating the harmful effects of ROS and, thus, protecting the spermatozoa. This study aimed to evaluate the effect of ellagic acid (EA) on the rabbit sperm traits after freezing-thawing. Semen samples collected from New Zealand White rabbit males were cryopreserved in a BotuCrio freezing medium (Nidacon, Sweden) supplemented with different concentrations of ellagic acid (EA at 0, 0.5, 1.5 and 2.5 mM) using the manual slow freezing procedure. After thawing, sperm motility parameters were evaluated by CASA. The parameters of viability (DRAQ7), apoptosis (Yo-Pro-1), acrosome integrity (peanut agglutinin; PNA), intracellular ROS (CellROX) and mitochondrial activity (MitoTracker) were evaluated by flow cytometry. EA added to the freezing medium at all concentrations led to a significant reduction (P < 0.05) in intracellular ROS in frozen-thawed sperm cells. However, this effect was not reflected in motility parameters. Semen supplemented with 1.5 mM EA also yielded a lower proportion of apoptotic cells compared to the control group. In conclusion, EA supplementation of semen extender demonstrated its antioxidative properties protecting spermatozoa against oxidative damage during cryopreservation. Nevertheless, to draw a definitive conclusion regarding the effect of EA on spermatozoa functionality, additional research is necessary.

Keywords: spermatozoa; antioxidant; viability; motility; ROS (search for similar items in EconPapers)
Date: 2024
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlcjs:v:69:y:2024:i:3:id:142-2023-cjas

DOI: 10.17221/142/2023-CJAS

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