Sucrase in immobilized cells of Cucumis sativus L

S.I. Ignatova, L.M. Kušč, L. Bilisics, L. Trinh, N.N. Tiuliaeva and M.G. Sedova
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S.I. Ignatova: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
L.M. Kušč: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
L. Bilisics: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
L. Trinh: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
N.N. Tiuliaeva: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia
M.G. Sedova: Selection & Seed Production Agrocompany "Iljinicha, Llc", Moscow, Russia

Horticultural Science, 2002, vol. 29, issue 1, 17-22

Abstract: Cell suspension cultures of Cucumis sativus L. - cucumber were permeabilized by Tween, hexadecyltri-methylammonium bromide, hexadecylpyridiniumbromide ethanol and/or immobilized by glutaraldehyde. The highest invertase activity was at pH 4.4 and temperature 53°C. The hydrolysis of the substrate was linear for 5 h reaching 60% conversion. The cells displayed high sucrase activity and convenient physico-mechanical properties.

Keywords: glutaraldehyde; sucrase; cell permeabilization; cell immobilization; Cucumis sativus L (search for similar items in EconPapers)
Date: 2002
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:29:y:2002:i:1:id:4465-hortsci

DOI: 10.17221/4465-HORTSCI

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