Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

I. Ajalin, F. Kobza and J. Doležel
Additional contact information
I. Ajalin: Mendel University of Agriculture and Forestry, Brno, Faculty of Horticulture, Lednice na Moravì, Czech Republic
F. Kobza: Mendel University of Agriculture and Forestry, Brno, Faculty of Horticulture, Lednice na Moravì, Czech Republic
J. Doležel: Mendel University of Agriculture and Forestry, Brno, Faculty of Horticulture, Lednice na Moravì, Czech Republic

Horticultural Science, 2002, vol. 29, issue 1, 35-40

Abstract: The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola × wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.

Keywords: flow cytometry; polyploidisation; colchicine; hexadecaploids; polyploidisation efficiency; morphological characteristics; Viola × wittrockiana Gams (search for similar items in EconPapers)
Date: 2002
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://hortsci.agriculturejournals.cz/doi/10.17221/4468-HORTSCI.html (text/html)
http://hortsci.agriculturejournals.cz/doi/10.17221/4468-HORTSCI.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:29:y:2002:i:1:id:4468-hortsci

DOI: 10.17221/4468-HORTSCI

Access Statistics for this article

Horticultural Science is currently edited by Ing. Eva Karská (Executive Editor)

More articles in Horticultural Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().