Identification of sucrase activity in cell suspension and culture medium of melon

J. Stano, K. Mičieta, A. Barth, M. Valšíková, M. Fulmeková, P. Matejka and M. Varadínová
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J. Stano: Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
K. Mičieta: Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic
A. Barth: Institute of Biochemistry, M. Luther University, Halle/Saale, Federal Republic of Germany
M. Valšíková: Research Institute of Vegetables, Nové Zámky, Slovak Republic
M. Fulmeková: Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
P. Matejka: Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic
M. Varadínová: Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic

Horticultural Science, 2005, vol. 32, issue 3, 104-107

Abstract: The activity of (soluble acid) sucrase was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple and rapid procedure for the identification and determination of extracellular sucrase from a culture medium of watermelon cell suspension cultures is described. Sucrose was used as a substrate for the determination of extracellular and intracellular activities of the enzyme. Intracellular activity was estimated from the cell suspension. The results show a 91.5-92.0% intracellular and 8.0-8.5% extracellular distribution of sucrase activity. The described method enables to carry out a rapid, simple and specific detection of extracellular sucrase in plants.

Keywords: intracellular sucrase; extracellular sucrase; watermelon (search for similar items in EconPapers)
Date: 2005
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:32:y:2005:i:3:id:3774-hortsci

DOI: 10.17221/3774-HORTSCI

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