Relative concentration of Apple mosaic virus coat protein in different parts of apple tree

J. Svoboda and J. Polák
Additional contact information
J. Svoboda: Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic
J. Polák: Division of Plant Health, Crop Research Institute, Prague-Ruzyně, Czech Republic

Horticultural Science, 2010, vol. 37, issue 1, 22-26

Abstract: The optimal conditions for detecting Apple mosaic virus were assessed by determining relative concentrations of viral coat protein in different tissues (leaves, flower petals, dormant buds, and phloem) in five selected symptomless Apple mosaic virus-infected apple trees of two cultivars at different terms during the vegetation period. Relative concentrations of Apple mosaic viru were calculated as the reciprocal value of the Apple mosaic virus coat protein titer determined by ELISA. The highest relative virus concentration and therefore the highest reliability of virus detection was obtained with young leaves in April before flowering. The reliability of the detection was proved by repeating tests of 80 apple trees of four cultivars in the two subsequent years. The presence of Apple mosaic virus was tested in young apple leaves before flowering taken from 472 apple trees from selected orchards of the Czech Republic. The association of the outcome with climate is also discussed.

Keywords: Apple mosaic virus (ApMV); ELISA; detection; virus titer; concentration; apple tree; leaf; flower; dormant bud; phloem; distribution; climate; orchard (search for similar items in EconPapers)
Date: 2010
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://hortsci.agriculturejournals.cz/doi/10.17221/39/2009-HORTSCI.html (text/html)
http://hortsci.agriculturejournals.cz/doi/10.17221/39/2009-HORTSCI.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:37:y:2010:i:1:id:39-2009-hortsci

DOI: 10.17221/39/2009-HORTSCI

Access Statistics for this article

Horticultural Science is currently edited by Ing. Eva Karská (Executive Editor)

More articles in Horticultural Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().