The elimination of Plum pox virus in plum cv. Bluefree and apricot cv. Hanita by chemotherapy of in vitro cultures

A. Hauptmanová and J. Polák
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A. Hauptmanová: Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic
J. Polák: Department of Virology, Crop Research Institute, Prague-Ruzyně, Czech Republic

Horticultural Science, 2011, vol. 38, issue 2, 49-53

Abstract: In vitro cultures of plum cv. Bluefree and apricot cv. Hanita infected with Plum pox virus (PPV) were used for the virus elimination by chemotherapy. Low ribavirin concentrations of 5 and 10 mg/l in Murashige-Skoog medium were applied in the treatment. Plum pox virus was completely eliminated by 5 mg/l of ribavirin in plum cv. Bluefree within twenty weeks and in apricot cv. Hanita in twelve weeks of the application. Plum pox virus was completely eliminated by 10 mg/l of ribavirin both in plum cv. Bluefree and apricot cv. Hanita within twelve weeks. The presence of PPV was not proved by RT-PCR. Clones of plum cv. Bluefree and apricot cv. Hanita were re-tested by RT-PCR one year after the termination of the ribavirin treatment and negative results confirmed the elimination of Plum pox virus.

Keywords: sharka; plant virus; chemotherapy; ribavirin; apricot; plum (search for similar items in EconPapers)
Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:38:y:2011:i:2:id:10-2010-hortsci

DOI: 10.17221/10/2010-HORTSCI

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