Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures: Short communication

J. Sedlák and F. Paprštein
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J. Sedlák: Research and Breeding Institute of Pomology Holovousy, Ltd., Hořice, Czech Republic
F. Paprštein: Research and Breeding Institute of Pomology Holovousy, Ltd., Hořice, Czech Republic

Horticultural Science, 2011, vol. 38, issue 4, 159-162

Abstract: The goal of this study was to determine an efficient micropropagation system for cranberry (Vaccinium macrocarpon Ait.). Cranberry cultivar Howes was successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson's rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested. Generally, the highest multiplication rate (2.7) was obtained for cv. Howes on AN medium with the concentration 1 mg/l of zeatin. The effects of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphtalen acetic acid (NAA) on root induction were tested in WPM medium. The percentage of rooting was 100% for NAA, 86% for IBA and 81% for IAA. From the tested variants, auxin NAA also promoted the highest development of good quality roots (more than 5) per shoot without callus formation.

Keywords: in vitro; multiplication; rooting; explant; Howes (search for similar items in EconPapers)
Date: 2011
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:38:y:2011:i:4:id:115-2010-hortsci

DOI: 10.17221/115/2010-HORTSCI

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