In vitro elimination of Black raspberry necrosis virus from black raspberry (Rubus occidentalis) - Short Communication

E.J. Cheong, A.R. Jeon, J.W. Kang, R. Mock, G. Kinard and R. Li
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E.J. Cheong: National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA
A.R. Jeon: National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA
J.W. Kang: National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA
R. Mock: National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA
G. Kinard: National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA
R. Li: National Germplasm Resources Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, USA

Horticultural Science, 2014, vol. 41, issue 2, 95-99

Abstract: Black raspberry necrosis virus (BRNV) is one of the most important viral pathogens of Rubus spp. In this study, a procedure combining in vitro culture and heat therapy on axillary buds of Rubusoccidentalis was developed to eliminate BRNV from infected plants. Axillary buds were grown aseptically at 4-h alternating periods of 29°C and 38°C with 14-h day length for 1-5 weeks. Shoots induced after thermotherapy were tested for the viruses by RT-PCR. BRNV was not detected in any shoots indicating that BRNV was heat labile. BRNV-free shoots were rooted in vitro and acclimatized in greenhouse. Regenerated plants remained virus-free after 2-month acclimatization, 3-month dormancy and 5-month growth periods.

Keywords: BRNV; Rubus occidentalis; axillary bud culture; thermotherapy (search for similar items in EconPapers)
Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:41:y:2014:i:2:id:266-2013-hortsci

DOI: 10.17221/266/2013-HORTSCI

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