Molecular S-genotyping of sweet cherry (Prunus avium L.) genetic resources

Josef Patzak, Alena Henychová, František Paprštein and Jiří Sedlák
Additional contact information
Josef Patzak: 2 Research and Breeding Institute of Pomology, Holovousy, Czech Republic *Corresponding author: patzak@chizatec.cz
Alena Henychová: 2 Research and Breeding Institute of Pomology, Holovousy, Czech Republic *Corresponding author: patzak@chizatec.cz
František Paprštein: 2 Research and Breeding Institute of Pomology, Holovousy, Czech Republic *Corresponding author: patzak@chizatec.cz
Jiří Sedlák: 2 Research and Breeding Institute of Pomology, Holovousy, Czech Republic *Corresponding author: patzak@chizatec.cz

Horticultural Science, 2019, vol. 46, issue 3, 146-152

Abstract: Sweet cherries are self-incompatible, which is determined by a gametophytic self-incompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic S-locus. Knowledge about the S-allele constitution of the cultivars is essential for fruit growers and breeders. Recently, molecular PCR-based methods have been developed to distinguish all S-alleles in sweet cherries. In our work, we analysed S-locus genotypes by 13 universal and allele-specific PCR primer combinations within 117 registered, old and local sweet cherry cultivars from the Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy, the Czech Republic. We confirmed the previous S-genotyping for 66 accessions except for Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Badeborner, Bing, Alfa, Gamma, Huldra, Rivan, Valerij Tschkalov, Viola and Winkler's Frühe. It could be due to either mislabelling or mistakes in the previous analyses. Newly, S-genotyping was determined for 51 accessions in which we found 4 new S-loci combinations. We detected the S-locus combinations in 19 incompatibility groups. The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6) with 22, 20, 12 and 12 genotypes, respectively.

Keywords: self-incompatibility; S-RNase alleles; incompatibility groups; cultivars (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
http://hortsci.agriculturejournals.cz/doi/10.17221/245/2017-HORTSCI.html (text/html)
http://hortsci.agriculturejournals.cz/doi/10.17221/245/2017-HORTSCI.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:46:y:2019:i:3:id:245-2017-hortsci

DOI: 10.17221/245/2017-HORTSCI

Access Statistics for this article

Horticultural Science is currently edited by Ing. Eva Karská (Executive Editor)

More articles in Horticultural Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().