Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism

Khethani Give Mhelembe, Johan Hendrik Kriel, Cecilia Bester, Rouvay Roodt-Wilding, Esmé Denise Louw and Kenneth Richard Tobutt
Additional contact information
Khethani Give Mhelembe: Agricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South Africa
Johan Hendrik Kriel: Agricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South Africa
Cecilia Bester: Agricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South Africa
Rouvay Roodt-Wilding: Department of Genetics, Stellenbosch University, Matieland, South Africa
Esmé Denise Louw: Department of Horticultural Science, Stellenbosch University, Matieland, South Africa
Kenneth Richard Tobutt: Agricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South Africa

Horticultural Science, 2020, vol. 47, issue 1, 69-79

Abstract: The ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars of domestic apple were analysed, 169 not previously genotyped. Of these, 82 were aa (homozygous for the high ethylene allele at 202 bp), 73 were ab and 14 bb (homozygous for the low ethylene allele, with the SINE, at 339 bp). The difference between the allele sizes, 137 bp, observed in the current study is consistent with the indel of 138 bp originally described, but differs considerably from the indel of 166 bp reported in literature. In addition, 21 accessions of other Malus species were analysed. Only one, M. 'Golden Hornet', had the b allele, which suggests it may have been introgressed from M. pumila.

Keywords: apple; fruit quality; genotyping; storability; Malus sp.; marker-assisted selection (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://hortsci.agriculturejournals.cz/doi/10.17221/83/2018-HORTSCI.html (text/html)
http://hortsci.agriculturejournals.cz/doi/10.17221/83/2018-HORTSCI.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:47:y:2020:i:1:id:83-2018-hortsci

DOI: 10.17221/83/2018-HORTSCI

Access Statistics for this article

Horticultural Science is currently edited by Ing. Eva Karská (Executive Editor)

More articles in Horticultural Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().