Storage of proliferating gooseberry cultures under slow growth conditions

Danuta Kucharska, Teresa Orlikowska, Robert Maciorowski, Małgorzata Kunka and Angelika Niewiadomska-Wnuk
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Danuta Kucharska: Research Institute of Horticulture, Skierniewice, Poland
Teresa Orlikowska: Research Institute of Horticulture, Skierniewice, Poland
Robert Maciorowski: Research Institute of Horticulture, Skierniewice, Poland
Małgorzata Kunka: Research Institute of Horticulture, Skierniewice, Poland
Angelika Niewiadomska-Wnuk: Research Institute of Horticulture, Skierniewice, Poland

Horticultural Science, 2021, vol. 48, issue 3, 134-140

Abstract: Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.

Keywords: Ribes grossularia L.; micropropagation; necrotic explants; storage (search for similar items in EconPapers)
Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:48:y:2021:i:3:id:142-2020-hortsci

DOI: 10.17221/142/2020-HORTSCI

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