Resolving the browning during the establishment of the in vitro propogation of Prunus avium cv. 'Fuchen'

Yinkai Xi, Biao Zeng, Hengyu Huang, Ye Wang and Pinhui Yang
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Yinkai Xi: Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
Biao Zeng: Medicinal Plants Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, P.R. China
Hengyu Huang: Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
Ye Wang: Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China
Pinhui Yang: Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, P.R. China

Horticultural Science, 2022, vol. 49, issue 1, 1-9

Abstract: Prunus avium cv. 'Fuchen' is a cultivated species with a high-profile economic benefit. Four media were used as a basal media equipped with four exogenous hormones to establish an intact regeneration system. Sodium thiosulfate (Na2S2O3) and active carbon (AC) improved the browning phenomenon. The results indicated that 1.0 g/L of AC and 30 g/L of Na2S2O3 were the best combination to inhibit the browning on the optimal basal medium - olive medium (OM) (followed by woody plant medium). The OM containing 1.5 mg/L of zeatin, 2.0 mg/L of α-naphtalenaecetic acid (NAA) and 0.5 mg/L of kinetin was beneficial for the germination and growth of the axillary buds. Green bud points were visible at the base of the swollen base after 25 days with a high proliferation coefficient (more than 7.50). The most optimal medium for rooting was 1/2 OM combined with 1.0 mg/L of NAA and 0.5 mg/L of indole-3-butyric-acid with a 100% rooting rate. The survival rate was up to 100% after 60 days with acclimatisation. Generally, a high-effective regeneration system was established, which provides a reference for keeping the excellent traits of the cultivar.

Keywords: sweet cherry 'Fuchen'; bud proliferation; basal medium; rapid propagation; browning inhibition (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:49:y:2022:i:1:id:51-2020-hortsci

DOI: 10.17221/51/2020-HORTSCI

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