Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

Monika Poniewozik, Marzena Parzymies and Paweł Szot
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Monika Poniewozik: Institute of Horticultural Production, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Lublin, Poland
Paweł Szot: Institute of Horticultural Production, Faculty of Horticulture and Landscape Architecture, University of Life Sciences in Lublin, Lublin, Poland

Horticultural Science, 2022, vol. 49, issue 1, 48-51

Abstract: Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

Keywords: lady slipper; orchid; micropropagation; phenolic compounds; tissue culture (search for similar items in EconPapers)
Date: 2022
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlhor:v:49:y:2022:i:1:id:68-2020-hortsci

DOI: 10.17221/68/2020-HORTSCI

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