In vitro regeneration of Pistacia vera L. from nodal explants

B. Benmahioul, M. Kaïd-Harche and F. Daguin
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B. Benmahioul: Department of Forest Resources, Faculty of Natural Sciences and Life, Earth Sciences and the Universe, University of Abou-Bekr Belkaïd Tlemcen, Tlemcen, Algeria
M. Kaïd-Harche: Department of Biotechnology, Faculty of Sciences, University of Sciences and Technology of Oran Mohamed Boudiaf, Oran, Algeria
F. Daguin: Research Institute of Horticulture and Seeds, University of Angers, Angers, France

Journal of Forest Science, 2016, vol. 62, issue 5, 198-203

Abstract: To enhance the induction of shoots, the excised nodes were cultured on Murashige and Skoog medium containing different cytokinins and various concentrations. The best conditions for shoot induction and growth were with 6-benzyladenine at 1.0 mg.l-1. For axillary shoot proliferation, the excellent result was obtained using 2.0 mg.l-1 meta-topoline. Well-developed shoots (more than 2 cm in length) were successfully rooted ex vitro at 82% by treatment with commercial rooting powder (2% indole-3-butyric acid; Rhizopon®). Rooted plantlets were acclimatized in the greenhouse and the survival rate of transplantation reached 80%.

Keywords: sterilizing agents; morphogenic response; cytokinin; shoot proliferation; ex vitro rooting; tissue culture (search for similar items in EconPapers)
Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnljfs:v:62:y:2016:i:5:id:82-2015-jfs

DOI: 10.17221/82/2015-JFS

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