Morphological variability between diploid and tetraploid taxa of the genus Betula L. in the Czech Republic

Rostislav Linda, Ivan Kuneš, Martin Baláš and Josef Gallo
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Ivan Kuneš: Department of Silviculture, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic
Martin Baláš: Department of Silviculture, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic
Josef Gallo: Department of Silviculture, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic

Journal of Forest Science, 2017, vol. 63, issue 12, 531-537

Abstract: The aim of this work was to suggest a reliable method for distinguishing between diploid and tetraploid taxa of the genus Betula Linnaeus, based on leaf measurements. In total, 97 individuals from 6 locations within the Bohemian Forest region (Czech Republic) were selected. Four leaves from each individual were evaluated. On each leaf, twenty parameters were measured. Each sample was analysed by flow cytometry to determine its actual ploidy. Measured parameters were analysed by principal component analysis and tested for differences between diploid and tetraploid taxa. For actual ploidy prediction, a classification function was designed. The reliability of the classification function was verified on samples from three different regions of the Czech Republic and compared with functions as suggested by other authors. The classification function designed in this work (based on 3 parameters - blade width in the upper 1/4 of blade length, first vein angle and number of leaf teeth between 3rd and 4th vein) correctly determined actual ploidy in 89% of all tested samples.

Keywords: silver birch; downy birch; ploidy; flow cytometry; classification function (search for similar items in EconPapers)
Date: 2017
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Citations: View citations in EconPapers (1)

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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnljfs:v:63:y:2017:i:12:id:105-2017-jfs

DOI: 10.17221/105/2017-JFS

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