Development of RT-PCR tests for the routine detection of latent and ILAR viruses in fruit trees

S. Marbot, J. Kummert, M. Salmon, M. Vendrame, A. Huwaert, O. Dutrecq and P. Lepoivre
Additional contact information
S. Marbot: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be
J. Kummert: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be
M. Salmon: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be
M. Vendrame: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be
A. Huwaert: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be
O. Dutrecq: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be
P. Lepoivre: Plant Pathology Unit, Agricultural University of Gembloux (FUSAGx), 5030 Gembloux, Belgium * Tel.: +32 81 622 431, Fax: +32 81 610 126, E-mail: roussel.s@fsagx.ac.be

Plant Protection Science, 2002, vol. 38, issue SI1-6thConfEFPP, S21-S23

Abstract: The detection throughout the year of latent and ILAR viruses in fruit trees by classical serological tests appears to be unreliable. Recently, these problems have smoothed themselves out by the use of molecular methods. We have developed RT-PCR protocols which are simple and reliable for routine detection of these viruses throughout the year.

Keywords: ACLSV; ASPV; ASGV; PDV; PNRSV; ApMV; fruit trees; RT-PCR; RT-PCR-ELOSA; Minor Groove Binder-DNA probe (search for similar items in EconPapers)
Date: 2002
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Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10312-pps

DOI: 10.17221/10312-PPS

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