Molecular identification of a phytoplasma naturally infecting Populus nigra L. cv. Italica trees in Croatia

M. Šeruga, D. Škorić, S. Botti, S. Paltrinieri, N. Juretić and A. Bertaccini
Additional contact information
M. Šeruga: Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia
D. Škorić: Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia
S. Botti: Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia
S. Paltrinieri: Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia
N. Juretić: Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia
A. Bertaccini: Department of Biology, Faculty of Science, University of Zagreb, HR-10000 Zagreb, Croatia

Plant Protection Science, 2002, vol. 38, issue SI1-6thConfEFPP, S28-S30

Abstract: Leaf and branch samples of 10 Populus nigra L. cv. Italica trees were collected from the urban Zagreb area in late summer/autumn 2001. One of the trees exhibited leaf yellowing, overall sparse foliage, stunting and decline. Phytoplasma 16S rDNA was amplified in direct and nested PCR assays using universal and specific phytoplasma primer pairs, from nucleic acids extracted by two different procedures. Strong amplification signals were observed in samples from symptomatic Lombardy poplar as well as in samples from 4 of the asymptomatic trees. RFLP analyses of amplicons showed patterns characteristic of the phytoplasmas belonging to the Aster yellows group (16SrI). This is the first report of a phytoplasma naturally infecting poplar in Croatia.

Keywords: aster yellows phytoplasma; lombardy poplar; PCR; RFLP; 16S rDNA (search for similar items in EconPapers)
Date: 2002
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
http://pps.agriculturejournals.cz/doi/10.17221/10314-PPS.html (text/html)
http://pps.agriculturejournals.cz/doi/10.17221/10314-PPS.pdf (application/pdf)
free of charge

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:caa:jnlpps:v:38:y:2002:i:si1-6thconfefpp:id:10314-pps

DOI: 10.17221/10314-PPS

Access Statistics for this article

Plant Protection Science is currently edited by Ing. Eva Karská, (Executive Editor PPS)

More articles in Plant Protection Science from Czech Academy of Agricultural Sciences
Bibliographic data for series maintained by Ivo Andrle ().