Identification and characterization of host proteins interacting with NSm, the Tomato spotted wilt virus movement protein

M. Paape, S. Nell, S. Von Bargen and J.-W. Kellmann
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M. Paape: Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany
S. Nell: Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany
S. Von Bargen: Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany
J.-W. Kellmann: Universität Rostock, Fachbereich Biowissenschaften, Abteilung Biochemie, D-18059 Rostock, Germany

Plant Protection Science, 2002, vol. 38, issue SI1-6thConfEFPP, S108-S111

Abstract: To search for host proteins involved in systemic spreading of Tomato spotted wilt virus (TSWV), the virus-encoded NSm movement protein has been utilized as a bait in yeast two-hybrid interaction trap assays. J-domain chaperones from different host species and a protein denominated At-4/1 from Arabidopsis thaliana showing homologies to myosins and kinesins were identified as NSm-interacting partners. In this communication we illustrate that following TSWV infection, J-domain proteins accumulated in systemically infected leaves of A. thaliana, whereas At-4/1 was constitutively detected in leaves of A. thaliana and Nicotiana rustica.

Keywords: Tomato spotted wilt virus (TSWV); Arabidopsis thaliana; Nicotiana rustica; movement protein; J-domain protein; yeast two-hybrid system (search for similar items in EconPapers)
Date: 2002
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DOI: 10.17221/10331-PPS

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