Selection of RNA isolation method for molecular detection of grapevine viruses

P. Komínek
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P. Komínek: Research Institute of Crop Production, 161 06 Prague-Ruzyně, Czech Republic

Plant Protection Science, 2002, vol. 38, issue SI2-6thConfEFPP, 267-270

Abstract: Grapevines infected with Grapevine leafroll-associated virus-1 (GLRaV-1) and Grapevine leafroll-associated virus-3 (GLRaV-3) were selected. Total RNA was isolated from grapevine phloem tissue scrapped from dormant canes by three different methods: extraction with urea buffer followed with phenol-chloroform extraction, method using ConcertTM reagent (Invitrogen) followed with chloroform-isopropylalcohol extraction, and procedure using RNeasy Plant Mini Kit (Qiagen). The highest yield of RNA was obtained using ConcertTM reagent. If this RNA was used in RT-PCR, GLRaV-1 and GLRaV-3 were easily detected. From RNA isolated by other two methods these viruses were not detected.

Keywords: Grapevine leafroll-associated virus-1; Grapevine leafroll-associated virus-3; grapevine; ELISA; RT-PCR (search for similar items in EconPapers)
Date: 2002
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DOI: 10.17221/10463-PPS

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